mAb 18.2, which recognises the Knob Associated Histidine Rich protein (KAHRP) is now available. Click the links in the product description to view technical data on the monoclonal antibody. Suitable for Western Blotting, IFA and confocal microscopy.
UPDATE: Recent publication on the use of mAb 18.2 in Blood;
WE have 8-week funded training opportunities are available for young scientists from malaria endemic countries. Expenses of £2000 can be provided for short visits to the repository lab in Edinburgh. Training will involve ELISA, GIA, IFA and parasite culture, depending on the requirements of the applicant. For more details, please contact the repository director, Dr. David Cavanagh firstname.lastname@example.org .
New monoclonal antibodies to the Plasmodium falciparum FIKK4.2 kinase are now available. A recent gift from Dr. Odile Mercereau-Puijalon, these antibodies recognise the R45 antigen, now known as FIKK4.2. See Kats et al., (2014) International Journal for Parasitology 44: 319–328.
Mike Blackman, NIMR, London, has kindly deposited the Plasmodium falciparum clone 1G5DiCre for DiCre-mediated gene knock-down and selectable marker recycling. This is a novel knockout system which can produce constitutively DiCre-expressing P. falciparum clones that have broad utility for the functional analysis of essential asexual blood-stage parasite genes. The plasmid pHH1-SERA5del3preDiCre is also available.
Dr. Colin Sutherland, LSHTM, UK, has deposited with the EMRR seven new culture-adapted Plasmodium falciparum lines from UK travellers. These are labelled HL1204 to HL1214 in the Plasmodium falciparum section of the reagents lists. All are polyclonal isolates of varying drug sensitivities, from five different African countries. We have expanded all of these lines. Vials of parasites can be shipped via our couriers worldwide.
Anti-GST (S. japonicum) is now available - a monoclonal antibody produced as a by-product of immunisation with P. falciparum GST fusion proteins, this is a useful reagent for detection of GST fusion proteins (WB, ELISA) produced using the pGEX (™) system. Cross-reactivity with P. falciparum GST unknown.
Three monoclonal antibodies to Plasmodium falciparum glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are now available as protein G purified reagents. mAbs to GAPDH are commonly used as a loading control for Western blots, and as a marker of the parasite cytoplasm. GAPDH is a highly abundant enzyme involved in the sixth step of glycolysis. Positive in IFA with P. vivax. May also be cross-reactive with GAPDH from rodent Plasmodia.
The European Malaria Reagent Repository launches its new website on 12/05/2013
The repository has a collection of parasite strains, clones and lines from both human and rodents, monoclonal antibodies to a wide range of blood stage asexual and gametocyte antigens, and a panel of ~60 recombinant antigens derived from blood stage expressed P. falciparum and P. chabaudi genes. We can also provide P. falciparum parasite extracts and DNA on request, as well as IFA slides.
Monoclonal antibody 7.7, specific for the EXP-2 protein, a component of the PTEX transport complex on the parasitophorous vacuole membrane, is now available from the repository. This antibody is useful for localisation of the parasite membrane in IFAT or confocal studies (see Bullen et al., (2012). J. Biol. Chem. 287: 7871
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